Simultaneous determination of four 5-hydroxy polymethoxyflavones by reversed-phase high performance liquid chromatography with electrochemical detection
Identifieur interne : 001C54 ( Main/Exploration ); précédent : 001C53; suivant : 001C55Simultaneous determination of four 5-hydroxy polymethoxyflavones by reversed-phase high performance liquid chromatography with electrochemical detection
Auteurs : PING DONG [États-Unis] ; PEIJU QIU [États-Unis, République populaire de Chine] ; YI ZHU [États-Unis, France] ; SHIMING LI [États-Unis] ; Chi-Tang Ho [États-Unis] ; David Julian Mcclements [États-Unis] ; HANG XIAO [États-Unis]Source :
- Journal of chromatography [ 0021-9673 ] ; 2010.
Descripteurs français
- Pascal (Inist)
- Mesure simultanée, Analyse chimique, Dérivé de la flavone, Chromatographie phase inverse, Chromatographie HPLC, Détecteur électrochimique, Analyse quantitative, Validation, Citrus sinensis, Jus d'orange, Pelure, Fruit, Citrus reticulata, Plante médicinale, Pharmacognosie, Orange, Flavonoïde, Mandarine, Origine végétale, Médecine traditionnelle chinoise.
- Wicri :
- topic : Analyse quantitative, Fruit, Plante médicinale.
English descriptors
- KwdEn :
- Chemical analysis, Citrus reticulata, Citrus sinensis, Electrochemical detector, Flavone derivatives, Flavonoid, Fruit, HPLC chromatography, Mandarin, Medicinal plant, Orange, Orange juice, Peel, Pharmacognosy, Plant origin, Quantitative analysis, Reversed phase chromatography, Simultaneous measurement, Validation.
Abstract
Accumulating evidence has suggested the potential health-promoting effects of 5-hydroxy polymethoxyflavones (5-OH-PMFs) naturally existing in citrus genus. However, research efforts are hampered by the lack of reliable and sensitive methods for their determination in plant materials and biological samples. Using reversed-phase high performance liquid chromatography (HPLC) equipped with electrochemical (EC) detection, we have developed a fast and highly sensitive method for quantification of four 5-OH-PMFs, namely 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone, 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone, 5-hydroxy-6,7,4'-trimethoxyflavone, and 5-hydroxy-6,7,8,4'-tetramethoxyflavone. The method was fully validated in terms of linearity, accuracy and precision. The limit of detection (LOD) was determined as being between 0.65 and 1.8ng/mL (ppb), demonstrating an over 160 times higher sensitivity in comparison with the previously reported method using UV detection. The recovery rate of the method was between 96.17% and 110.82%, and the precision for the retention times and peak areas was all below 13%. The method was successfully used to quantify 5-OH-PMFs with a wide range of abundance in the citrus products and preparations, such as orange juice, citrus peel, and dried tangerine peel. The quantification method for 5-OH-PMFs developed herein could be useful for the nutritional and pharmacological studies of these compounds in future.
Affiliations:
- France, République populaire de Chine, États-Unis
- Bourgogne, Bourgogne-Franche-Comté, Massachusetts, New Jersey
- Amherst (Massachusetts), New Brunswick (New Jersey)
- Université Rutgers, Université du Massachusetts
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electrochemical detection</title><author><name sortKey="Ping Dong" sort="Ping Dong" uniqKey="Ping Dong" last="Ping Dong">PING DONG</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Food Science, University of Massachusetts, 100 Holdworth Way</s1><s2>Amherst, MA 01003</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Massachusetts</region><settlement type="city">Amherst (Massachusetts)</settlement></placeName><orgName type="university">Université du Massachusetts</orgName></affiliation></author><author><name sortKey="Peiju Qiu" sort="Peiju Qiu" uniqKey="Peiju Qiu" last="Peiju Qiu">PEIJU QIU</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Food Science, University of Massachusetts, 100 Holdworth Way</s1><s2>Amherst, MA 01003</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Massachusetts</region><settlement type="city">Amherst (Massachusetts)</settlement></placeName><orgName type="university">Université du Massachusetts</orgName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Marine Drug and Food Institute, Ocean University of China, No. 5, Yu Shan Road</s1><s2>Qingdao, Shandong Province, 266003</s2><s3>CHN</s3><sZ>2 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Qingdao, Shandong Province, 266003</wicri:noRegion></affiliation></author><author><name sortKey="Yi Zhu" sort="Yi Zhu" uniqKey="Yi Zhu" last="Yi Zhu">YI ZHU</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Food Science, University of Massachusetts, 100 Holdworth Way</s1><s2>Amherst, MA 01003</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Massachusetts</region><settlement type="city">Amherst (Massachusetts)</settlement></placeName><orgName type="university">Université du Massachusetts</orgName></affiliation><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>National Superior School of Applied Biology to Nutrition and Food, University of Burgundy, 1 Esplanade Erasme</s1><s2>Dijon, 21000</s2><s3>FRA</s3><sZ>3 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Bourgogne-Franche-Comté</region><region type="old region" nuts="2">Bourgogne</region></placeName></affiliation></author><author><name sortKey="Shiming Li" sort="Shiming Li" uniqKey="Shiming Li" last="Shiming Li">SHIMING LI</name><affiliation wicri:level="2"><inist:fA14 i1="04"><s1>Analytical and Natural Product Chemistry, WellGen, Inc., 675 US Highway One</s1><s2>North Brunswick, NJ 08902</s2><s3>USA</s3><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">New Jersey</region></placeName></affiliation></author><author><name sortKey="Ho, Chi Tang" sort="Ho, Chi Tang" uniqKey="Ho C" first="Chi-Tang" last="Ho">Chi-Tang Ho</name><affiliation wicri:level="4"><inist:fA14 i1="05"><s1>Department of Food Science, Rutgers University, 65 Dudley Road</s1><s2>New Brunswick, NJ 08901-8520</s2><s3>USA</s3><sZ>5 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">New Jersey</region><settlement type="city">New Brunswick (New Jersey)</settlement></placeName><orgName type="university">Université Rutgers</orgName></affiliation></author><author><name sortKey="Mcclements, David Julian" sort="Mcclements, David Julian" uniqKey="Mcclements D" first="David Julian" last="Mcclements">David Julian Mcclements</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Food Science, University of Massachusetts, 100 Holdworth Way</s1><s2>Amherst, MA 01003</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Massachusetts</region><settlement type="city">Amherst (Massachusetts)</settlement></placeName><orgName type="university">Université du Massachusetts</orgName></affiliation></author><author><name sortKey="Hang Xiao" sort="Hang Xiao" uniqKey="Hang Xiao" last="Hang Xiao">HANG XIAO</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Food Science, University of Massachusetts, 100 Holdworth Way</s1><s2>Amherst, MA 01003</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Massachusetts</region><settlement type="city">Amherst (Massachusetts)</settlement></placeName><orgName type="university">Université du Massachusetts</orgName></affiliation></author></analytic><series><title level="j" type="main">Journal of chromatography</title><title level="j" type="abbreviated">J. chromatogr.</title><idno type="ISSN">0021-9673</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of chromatography</title><title level="j" type="abbreviated">J. chromatogr.</title><idno type="ISSN">0021-9673</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chemical analysis</term><term>Citrus reticulata</term><term>Citrus sinensis</term><term>Electrochemical detector</term><term>Flavone derivatives</term><term>Flavonoid</term><term>Fruit</term><term>HPLC chromatography</term><term>Mandarin</term><term>Medicinal plant</term><term>Orange</term><term>Orange juice</term><term>Peel</term><term>Pharmacognosy</term><term>Plant origin</term><term>Quantitative analysis</term><term>Reversed phase chromatography</term><term>Simultaneous measurement</term><term>Validation</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Mesure simultanée</term><term>Analyse chimique</term><term>Dérivé de la flavone</term><term>Chromatographie phase inverse</term><term>Chromatographie HPLC</term><term>Détecteur électrochimique</term><term>Analyse quantitative</term><term>Validation</term><term>Citrus sinensis</term><term>Jus d'orange</term><term>Pelure</term><term>Fruit</term><term>Citrus reticulata</term><term>Plante médicinale</term><term>Pharmacognosie</term><term>Orange</term><term>Flavonoïde</term><term>Mandarine</term><term>Origine végétale</term><term>Médecine traditionnelle chinoise</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Analyse quantitative</term><term>Fruit</term><term>Plante médicinale</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Accumulating evidence has suggested the potential health-promoting effects of 5-hydroxy polymethoxyflavones (5-OH-PMFs) naturally existing in citrus genus. However, research efforts are hampered by the lack of reliable and sensitive methods for their determination in plant materials and biological samples. Using reversed-phase high performance liquid chromatography (HPLC) equipped with electrochemical (EC) detection, we have developed a fast and highly sensitive method for quantification of four 5-OH-PMFs, namely 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone, 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone, 5-hydroxy-6,7,4'-trimethoxyflavone, and 5-hydroxy-6,7,8,4'-tetramethoxyflavone. The method was fully validated in terms of linearity, accuracy and precision. The limit of detection (LOD) was determined as being between 0.65 and 1.8ng/mL (ppb), demonstrating an over 160 times higher sensitivity in comparison with the previously reported method using UV detection. The recovery rate of the method was between 96.17% and 110.82%, and the precision for the retention times and peak areas was all below 13%. The method was successfully used to quantify 5-OH-PMFs with a wide range of abundance in the citrus products and preparations, such as orange juice, citrus peel, and dried tangerine peel. The quantification method for 5-OH-PMFs developed herein could be useful for the nutritional and pharmacological studies of these compounds in future.</div></front></TEI><affiliations><list><country><li>France</li><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Bourgogne</li><li>Bourgogne-Franche-Comté</li><li>Massachusetts</li><li>New Jersey</li></region><settlement><li>Amherst (Massachusetts)</li><li>New Brunswick (New Jersey)</li></settlement><orgName><li>Université Rutgers</li><li>Université du Massachusetts</li></orgName></list><tree><country name="États-Unis"><region name="Massachusetts"><name sortKey="Ping Dong" sort="Ping Dong" uniqKey="Ping Dong" last="Ping Dong">PING DONG</name></region><name sortKey="Hang Xiao" sort="Hang Xiao" uniqKey="Hang Xiao" last="Hang Xiao">HANG XIAO</name><name sortKey="Ho, Chi Tang" sort="Ho, Chi Tang" uniqKey="Ho C" first="Chi-Tang" last="Ho">Chi-Tang Ho</name><name sortKey="Mcclements, David Julian" sort="Mcclements, David Julian" uniqKey="Mcclements D" first="David Julian" last="Mcclements">David Julian Mcclements</name><name sortKey="Peiju Qiu" sort="Peiju Qiu" uniqKey="Peiju Qiu" last="Peiju Qiu">PEIJU QIU</name><name sortKey="Shiming Li" sort="Shiming Li" uniqKey="Shiming Li" last="Shiming Li">SHIMING LI</name><name sortKey="Yi Zhu" sort="Yi Zhu" uniqKey="Yi Zhu" last="Yi Zhu">YI ZHU</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Peiju Qiu" sort="Peiju Qiu" uniqKey="Peiju Qiu" last="Peiju Qiu">PEIJU QIU</name></noRegion></country><country name="France"><region name="Bourgogne-Franche-Comté"><name sortKey="Yi Zhu" sort="Yi Zhu" uniqKey="Yi Zhu" last="Yi Zhu">YI ZHU</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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